(:source 
  :version  3 
  :ip-address "130.251.201.2"
  :ip-name "istge.ist.unige.it"
  :tcp-port 210
  :database-name "Cell_Lines"
  :source-name "/wais/cldb/Cell_Lines"
  :cost 0.00 
  :cost-unit :free 
  :maintainer "wais@istge.ist.unige.it"
  :subject "biology biotechnology bioinformatics"
  :description "Server created with WAIS release 8 b4 on Oct 13 1992 
                 Server updated with WAIS release 8 b5 on Feb 17 1993

Keywords: molecular biology, computational biology, cellular biology,
          cell cultures, culture collections, biocomputing, bioinformatics

At istge.ist.unige.it three databases devoted to availability of biological
materials are maintained and updated, in the sphere of the Interlab Project.
You can have access to them also by connecting to istge.ist.unige.it using 
telnet. A Gopher server was also created, use the following link:

Name=National Institute for Cancer Research (IST, Genova, Italy)
Type=1
Path=
Port=70
Host=istge.ist.unige.it

Ask gophman@istge.ist.unige.it for details.

Cell Line Data Base (CLDB) refers to animal cell lines availability in 
European research laboratories. 
Its WAIS version is also called Cell_Lines.
It currently includes data on more than 2600 cell lines from many Italian 
labs and from five of the most important European cell cultures collections.

Namely, there are data from:

- the European Collection of Animal Cell Cultures (ECACC), Salisbury (UK);
- the German Collection of Microorganisms and Cell Cultures (DSM), 
  Braunschweig (D);
- the Institute of Cytology, St. Peterburg (Russia);
- the Institute of Oncology and Radiology, Beograd (YU);

and, in Italy:

- the Center for the Study of Connective Tissue Diseases, Pavia (I);
- the Istituto Zooprofilattico Sperimentale, Brescia (I);
- the Giannina Gaslini Institute, Genoa (I);
- the Mario Negri Institute for Pharmaceutical Research, Milan (I);
- the Istituto Superiore di Sanita', Rome (I);
- the Institute for Biochemical and Evolutionary Genetics, Pavia (I);

and the following companies:

- SCLAVO SpA, Siena (I);
- ENEA SpA, Rome (I);
- OPOCRIN SpA, Modena (I);
- Farmitalia Carlo Erba SpA, Milan (I);
- Boehringer Mannheim Italia SpA, Monza (I);
- Bayer Italia SpA, Garbagnate Milanese (I);

Here is a sample record taken from CLDB:

293 (kidney, embryonic, human)
Continuous culture, grown as monolayer
epithelial-like morphology
Species: human 
contain and express the transforming genes of Ad5
Tissue/organ: kidney, embryonic
Transformed by: adenovirus 5
Susceptibility to viruses: adenovirus * astrovirus
Functions, products and applications:
virology * transformation * virus titration
Availability and advices:
- PHLS Centre for Applied Microbiology & Research (ECACC, Salisbury)
  Culture medium: EM30; Freezing medium: CME1; Split 1:2-3 Passage 36
  Validated by: isoenzymes (species confirmation)
  Seed at 3-5 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA. Detach at
  room temp.; may take many days to reattach. Can be used to isolate
  transformation defective host-range mutants of Ad5 and for titrating human
  adenoviruses
- Russian Academy of Medical Sciences (MWIIW, Moscow)
- Istituto Superiore di Sanita' (RMSUS, Roma)
  Culture medium: ME27; Freezing medium: CM01
- Russian Academy of Sciences (SPBIC, S. Peterburg)
Availability in commercial catalogues:
ATCC: CRL 1573;  ECACC: 85120602
Bibliographic references:
J. Gen. Virol. 1977, 36:59
Virology 1977, 77:319

Complete data on a particular laboratory can be retrieved by searching 
CLDB with a term composed by the laboratory's code plus the fixed postfix 
LAB (e.g., searching SPBICLAB will retrieve complete data relative to 
the SPBIC laboratory).

The description of a particular culture medium can be retrieved by 
searching CLDB with a term composed by the medium's code plus the fixed 
postfix CM (e.g., searching ME27CM will retrieve complete data relative 
to the ME27 culture medium).

The description of a particular freezing medium can be retrieved by 
searching CLDB with a term composed by the medium's code plus the fixed 
postfix FM (e.g., searching CM01FM will retrieve complete data relative 
to the CM01 freezing medium).

B Line Data Base (BLDB) refers to HLA typed B lymphoblastoid cell lines.
It includes data on typing of ca. 1400 lines from the European Collection 
for Biomedical Research (ECBR, Essen, Germany, and Genoa, Italy), 
the UK Transplant Service of Bristol and the European Collection of Animal 
Cell Cultures, Salisbury, UK.

Molecular Probe Data Base (MPDB) refers to oligonucleotides of up to 100
nucleotides. It includes data on ca. 1700 oligos.
"
  )
